Expression of Cholesteryl Ester Transfer Protein in Mice Promotes Macrophage Reverse Cholesterol Transport

Expression of cholesteryl ester transfer protein in mice promotes macrophage reverse cholesterol transport.

BACKGROUND Cholesteryl ester transfer protein (CETP) transfers cholesteryl esters from high-density lipoproteins to apolipoprotein (apo) B-containing lipoproteins and in humans plays an important role in lipoprotein metabolism. However, the role that CETP plays in mediation of reverse cholesterol transport (RCT) remains unclear. We used a validated in vivo assay of macrophage RCT to test the ef...

Cholesteryl ester transfer protein inhibition: effect on reverse cholesterol transport?

Statins, inhibitors of the key enzyme in the biosynthesis of cholesterol (ie, 3-hydroxy-3-methylglutaryl [HMG]coenzyme A [CoA] reductase), are widely used as the prevailing strategy to combat atherosclerosis through reducing LDL cholesterol levels. Large-scale clinical trials have shown that statins markedly reduce coronary events.1,2 A meta-analysis of 22 studies enrolling nearly 70 000 indivi...

The effect of cholesteryl ester transfer protein overexpression and inhibition on reverse cholesterol transport.

AIMS Cholesteryl ester transfer protein (CETP) has a well-established role in lipoprotein metabolism, but the effect of its overexpression or inhibition on the efficiency of reverse cholesterol transport (RCT) is unclear. METHODS AND RESULTS Neither overexpression of CETP nor treatment with CETP inhibitor Torcetrapib of RAW 264.7 macrophages or HepG2 hepatocytes affected cholesterol efflux in...

Antisense Oligonucleotide Inhibition of Cholesteryl Ester Transfer Protein Enhances Reverse Cholesterol Transport in Hyperlipidemic CETP Transgenic, LDLr-/- Mice

Fish oil promotes macrophage reverse cholesterol transport in mice.

OBJECTIVE Fish oil (FO), and specifically omega 3 fatty acids, has favorable effects on cardiovascular outcomes. The aim of this study was to investigate the effects of FO on the process of macrophage reverse cholesterol transport (RCT) in an in vivo mouse model. METHODS AND RESULTS C57BL/6J mice were fed a FO diet, whereas control mice were fed diets containing alternative sources of fats, s...